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Volume 13, Issue 5, Pages 304-309 (9 May 2006)


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Identification of equol producers in a Japanese population by high-performance liquid chromatography with coulometric array for determining serum isoflavones

J.H. Zhaoabc, S.J. Sunacd, Y. Araoac, E. Ogumaac, K. Yamadace, H. Horiguchiac, F. KayamaacCorresponding Author Informationemail address

Abstract 

Using a method of high-performance liquid chromatography (HPLC) with coulometric array, we measured isoflavone levels in sera from seven volunteers before and after three days of ingesting Soyaflavone E (an isoflavones powder) and from 129 female farmers (Japanese Multiple Environmental Toxicants Study; JMETS). Results showed that the serum isoflavone concentrations rose dramatically after three days of ingesting Soyaflavone E in all subjects except for the serum equol concentrations in two subjects. The geometric mean concentrations of daidzein, genistein, and equol in the serum of 129 Japanese women were 25.0ng/ml of daidzein, 94.1ng/ml of genistein, and 9.6ng/ml of equol. Interestingly, there existed two dominant groups in terms of serum equol concentrations in an independent manner of soy-derived product intake among the study participants.

a Center for Community Medicine, Department of Environmental Medicine, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan

b Second Hospital, Hebei Medical University, Shijiazhuang, China

c CREST-JST, Kawaguchi, Saitama, Japan

d College of Public Health, Hebei Medical University, Shijiazhang, China

e Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Japan

Corresponding Author InformationCorresponding author. Center for Community Medicine, Department of Environmental Medicine, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan. Tel.: +81285587336; fax: +81285448465.

 Core Research for Evolutionary Science and Technology, Japan Science Technology Corporation (CREST-JST) support this work.

PII: S0944-7113(05)00208-4

doi:10.1016/j.phymed.2005.09.013


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