Phytomedicine
Volume 16, Issue 1 , Pages 65-71 , January 2009

Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography–tandem mass spectrometry

  • Jeevan K. Prasain

      Affiliations

    • Department of Pharmacology & Toxicology, 456 McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • Corresponding Author InformationCorresponding author. Tel.: +12059962612; fax: +12059346944.
    • ,
  • Ning Peng

      Affiliations

    • Department of Cell Biology, 456 McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • ,
  • Ray Moore

      Affiliations

    • Comprehensive Cancer Center Mass Spectrometry Shared Facility, 456 McCallum Research Building, University of Alabama at Birmingham, Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • ,
  • Alireza Arabshahi

      Affiliations

    • Department of Pharmacology & Toxicology, 456 McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • ,
  • Stephen Barnes

      Affiliations

    • Department of Pharmacology & Toxicology, 456 McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • Comprehensive Cancer Center Mass Spectrometry Shared Facility, 456 McCallum Research Building, University of Alabama at Birmingham, Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA
    • ,
  • J. Michael Wyss

      Affiliations

    • Department of Cell Biology, 456 McCallum Research Building, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA

References 

  1. Brownsill R, Combal JP, Taylor A, Bertrand M, Luijten W, Walther B. The application of electrospray and neutral-loss mass spectrometry to the identification of the metabolites of S12813 in rat liver slices. Rapid Commun. Mass Spectrom. 1994;8:361–365
  2. Clarke DB, Lloyd AS, Botting NP, Oldfield MF, Needs PW, Wiseman H. Measurement of intact sulfate and glucuronide phytoestrogen conjugates in human urine using isotope dilution liquid chromatography–tandem mass spectrometry with [13C(3)]isoflavone internal standards. Anal. Biochem. 2002;309:158–172
  3. de Boer VC, Dihal AA, van der Woude H, Arts IC, Wolffram S, Alink GM, et al. Tissue distribution of quercetin in rats and pigs. J. Nutr. 2005;135:1718–1725
  4. Fang N, Yu S, Badger TM. Characterization of isoflavones and their conjugates in female rat urine using LC/MS/MS. J. Agric. Food Chem. 2002;50:2700–2707
  5. Hsu FL, Liu IM, Kuo DH, Chen WC, Su HC, Cheng JT. Antihyperglycemic effect of puerarin in streptozotocin-induced diabetic rats. J. Nat. Prod. 2003;66:788–792
  6. Keyler DE, Baker JI, Lee DY, Overstreet DH, Boucher TA, Lenz SK. Toxicity study of an antidipsotropic Chinese herbal mixture in rats: NPI-028. J. Altern. Complement. Med. 2002;8:175–183
  7. Li Y, Pan WS, Chen SL, Xu HX, Yang DJ, Chan AS. Pharmacokinetic, tissue distribution, and excretion of puerarin and puerarin–phospholipid complex in rats. Drug Dev. Ind. Pharm. 2006;32:413–422
  8. Lafaye A, Junot C, Ramounet-Le Gall B, Fritsch P, Ezan E, Tabet JC. Profiling of sulfoconjugates in urine by using precursor ion and neutral loss scans in tandem mass spectrometry. application to the investigation of heavy metal toxicity in rats. J. Mass Spectrom. 2004;39:655–664
  9. Meezan E, Meezan EM, Jones K, Moore R, Barnes S, Prasain JK. Contrasting effects of puerarin and daidzin on glucose homeostasis in mice. J. Agric. Food Chem. 2005;53:8760–8767
  10. Prasain JK, Jones K, Brissie N, Moore R, Wyss JM, Barnes S. Identification of puerarin and its metabolites in rats by liquid chromatography–tandem mass spectrometry. J. Agric. Food Chem. 2004;52:3708–3712
  11. Prasain JK, Xu J, Kirk M, Smith JM, Sfakianos J, Barnes S. Differential biliary excretion of genistein metabolites following intraduodenal and intravenous infusion of genistin in female rats. J. Nutr. 2006;136:2975–2979
  12. Prasain J, Peng N, Acosta E, Moore R, Arabshahi A, Meezan E, et al. Pharmacokinetic study of puerarin in rat serum by liquid chromatography tandem mass spectrometry. Biomed. Chromatogr. 2007;21:410–414
  13. Rimbach G, De Pascual-Teresa S, Ewins BA, Matsugo S, Uchida Y, Minihane AM, et al. Antioxidant and free radical scavenging activity of isoflavone metabolites. Xenobiotica. 2003;33:913–925
  14. Sieveking DP, Woo KS, Fung KP, Lundman P, Nakhla S, Celermajer DS. Chinese herbs Danshen and Gegen modulate key early atherogenic events in vitro. Int. J. Cardiol. 2005;105:40–45
  15. Sfakianos J, Coward L, Kirk M, Barnes S. Intestinal uptake and biliary excretion of the isoflavone genistein in rats. J. Nutr. 1997;127:1260–1268
  16. Turner R, Baron T, Wolffram S, Minihane AM, Cassidy A, Rimbach G, et al. Effect of circulating forms of soy isoflavones on the oxidation of low density lipoprotein. Free Radic. Res. 2004;38:209–216
  17. Wu L, Qiao H, Li Y, Li L. Protective roles of puerarin and Danshensu on acute ischemic myocardial injury in rats. Phytomedicine. 2007;14:652–658
  18. Xiong FL, Sun XH, Gan L, Yang XL, Xu HB. Puerarin protects rat pancreatic islets from damage by hydrogen peroxide. Eur. J. Pharmacol. 2006;529:1–7
  19. Xu XH, Zhao TQ. Effects of puerarin on d-galactose-induced memory deficits in mice. Acta Pharmacol. Sin. 2002;23:587–590
  20. Yasuda T, Kano Y, Saito K, Ohsawa K. Urinary and biliary metabolites of daidzin and daidzein in rats. Biol. Pharm. Bull. 1994;17:1369–1374
  21. Yu Z, Gao X, Zhao Y, Bi K. Determination of safflor yellow A, puerarin, ferulic acid, ginsenoside Rg1, and Rb1 in the Traditional Chinese Medicinal preparation Naodesheng injection by high-performance liquid chromatography. J. Chromatogr. Sci. 2006;44:272–275
  22. Zhang JY, Wang Y, Dudkowski C, Yang D, Chang M, Yuan J, et al. Characterization of metabolites of Celecoxib in rabbits by liquid chromatography/tandem mass spectrometry. J. Mass Spectrom. 2000;35:1259–1270
  23. Zhu JH, Wang XX, Chen JZ, Shang YP, Zhu JH, Guo XG, et al. Effects of puerarin on number and activity of endothelial progenitor cells from peripheral blood. Acta Pharmacol. Sin. 2004;25:1045–1051

PII: S0944-7113(08)00168-2

doi: 10.1016/j.phymed.2008.09.004

Phytomedicine
Volume 16, Issue 1 , Pages 65-71 , January 2009

Article Tools

* Image Usage & Resolution