Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract

Prasain, Jeevan K.; Peng, Ning; Dai, Yanying; Moore, Ray; Arabshahi, Alireza; Wilson, Landon; Barnes, Stephen; Michael Wyss, J.; Kim, Helen; Watts, Ray L.

doi:10.1016/j.phymed.2008.08.006

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Volume 16, Issue 2 , Pages 233-243, March 2009

Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract

Jeevan K. Prasain
- Departments of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Purdue-UAB Botanicals Center for Age-Related Disease, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Corresponding author at: Department of Pharmacology & Toxicology, MCLM 456, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA. Tel.: +12059962612; fax: +12059346944.
Ning Peng
- Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Yanying Dai
- Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Ray Moore
- Comprehensive Cancer Center Proteomics/Mass Spectrometry Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Alireza Arabshahi
- Departments of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Landon Wilson
- Comprehensive Cancer Center Proteomics/Mass Spectrometry Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Stephen Barnes
- Departments of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Purdue-UAB Botanicals Center for Age-Related Disease, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Comprehensive Cancer Center Proteomics/Mass Spectrometry Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA
J. Michael Wyss
- Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Purdue-UAB Botanicals Center for Age-Related Disease, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Helen Kim
- Departments of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
- Purdue-UAB Botanicals Center for Age-Related Disease, University of Alabama at Birmingham, Birmingham, AL 35294, USA
Ray L. Watts
- Department of Neurology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

Abstract

Proanthocyanidin rich plant extracts derived from grape seed extract (GSE), hawthorn and cranberry are on markets for their preventive effects against cardiovascular diseases and uroinfections in woman. However, the importance of these health beneficial effects of these botanicals remains elusive due to incomplete understanding of uptake, metabolism and bioavailability of proanthocyanidins in vivo. In the present study rats were given GSE orally (300mg/kg, twice a day) and blood and urine were collected over a 24h period. Monomeric catechins and their methylated metabolites, and proanthocyanidins up to trimers were detected in blood samples treated with GSE using LC-MS/MS operating in the multiple reaction monitoring (MRM) mode. A new tetramethylated metabolite of dimeric proanthocyanidin (m/z 633) in GSE-treated urine was tentatively identified. Using LC-MS/MS, (+)-catechin and (−)-epicatechin were identified in the brain conclusively. These data suggested that GSE catechins cross the blood brain barrier and may be responsible for the neuroprotective effects of GSE.

Keywords: Grape seed proanthocyanidins, Metabolism, LC-MS/MS, Brain