Phytomedicine
Volume 16, Issue 5 , Pages 416-425 , May 2009

Hypoglycemic effect of polysaccharide enriched extract of Astragalus membranaceus in diet induced insulin resistant C57BL/6J mice and its potential mechanism

  • Xian-qing Mao

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
    • These authors contributed equally to this work.
  • ,
  • Feng Yu

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
    • These authors contributed equally to this work.
  • ,
  • Nian Wang

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
    • These authors contributed equally to this work.
  • ,
  • Yong Wu

      Affiliations

    • Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma 73104, USA
  • ,
  • Feng Zou

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
  • ,
  • Ke Wu

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
  • ,
  • Min Liu

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
  • ,
  • Jing-ping Ouyang

      Affiliations

    • Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, 115# Donghu Road, Wuhan 430071, China
    • Corresponding Author InformationCorresponding author. Tel.:+862768759846; fax:+862787331077.

References 

  1. Ahmad F, Azevedo JL, Cortright J, et al. Alterations in skeletal muscle protein-tyrosine phosphatase activity and expression in insulin-resistant human obesity and diabetes. J. Clin. Invest. 1997;100(2):449–458
  2. Alford FP, Martin FIR, Pearson MJ. Significance and interpretation of mildly abnormal oral glucose tolerance test. Diabetologia. 1971;7:173–180
  3. Berge RK, Tronstad KJ, Berge K, et al. The metabolic syndrome and the hepatic fatty acid drainage hypothesis. Biochimie. 2005;87:15–20
  4. Bonora E, Targher G, Alberiche M, et al. Homeostasis model assessment closely mirrors the glucose clamp technique in the assessment of insulin sensitivity studies in subjects with various degrees of glucose tolerance and insulin sensitivity. Diabetes Care. 2000;23:57–63
  5. Calfon M, Zeng H, Urano F, et al. IRE1 couples endoplasmic reticulum load to secretary capacity by processing the XBP-1 mRNA. Nature. 2002;415(6867):92–96
  6. Deborah MM, Christopher BN. Insulin resistance takes a trip through the ER. Science. 2004;306:425–426
  7. Eriksson S, Eriksson KF, Bondesson L. Nonalcoholic steatohepatitis in obesity: a reversible condition. Acta Med. Scand. 1986;20(1):83–88
  8. Fawaz GH, Janice MZ, Young BK, et al. Liver-specific protein-tyrosine phosphatase 1B (PTP1B) Re-expression alters glucose homeostasis of PTP1B-/- mice. J. Biol. Chem. 2005;280(15):15038–15046
  9. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 1957;226(1):497–509
  10. Gu F, Nguyen DT, Stuible M, et al. Protein-tyrosine phosphatase 1B potentiates IRE1 signaling during endoplasmic reticulum stress. J. Biol. Chem. 2004;279(48):49689–49693
  11. Goldstein BJ. Protein-tyrosine phosphatase 1B (PTP1B): a novel therapeutic target for type 2 diabetes mellitus, obesity and related states of insulin resistance. Curr. Drug Targets Immune Endocr. Metab. Disord. 2001;1(3):265–275
  12. Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J. Ethnopharmacol. 2002;81(1):81–100
  13. Grulet H, Duriach V, Hecart AC, et al. Study of the rate of early glucose disappearance following insulin injection, insulin sensitivity index. Diabetes Res. Clin. Pract. 1993;20:207–210
  14. Hofmann SM, Dong HJ, Li Z, et al. Improved insulin sensitivity is associated with restricted intake of dietary glycoxidation products in the db/db mouse. Diabetes. 2002;51(7):2082–2089
  15. Issad T, Boute N, Boubekeur S, et al. Interaction of PTP1B with the insulin receptor precursor during its biosynthesis in the endoplasmic reticulum. Biochimie. 2005;87(1):111–116
  16. Iwawaki T, Akai R. Analysis of the XBP1 splicing mechanism using endoplasmic reticulum stress-indicators. Biochem. Biophys. Res. Commun. 2006;350(3):709–715
  17. Lee AH, Iwakoshi NN, Glimcher LH. XBP-1 regulates a subset of endoplasmic reticulum resident chaperone genes in the unfolded protein response. Mol. Cell Biol. 2003;23(21):7448–7459
  18. Mao CP, Xie ML, Gu ZL. Effects of konjac extract on insulin sensitivity in high fat diet rats. Acta Pharmacol. Sin. 2002;23:855–859
  19. Mao XQ, Wu Y, Wu K, et al. Astragalus polysaccharide reduces hepatic endoplasmic reticulum stress and restores glucose homeostasis in a diabetic KKAy mouse model. Acta Pharmacol. Sin. 2007;28(12):1947–1956
  20. Ni Y, Su Q, Liu X, et al. Experimental study of optimized techniques of water decoction extraction of Astragalus polysaccharide. Zhongguo Zhong Yao Za Zhi. 1998;23:284–286
  21. Ozcan U, Cao Q, Yilmaz E, Lee AH, Iwakoshi NN, Ozdelen E, et al. Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes. Science. 2004;306(5695):457–461
  22. Paula AK. A surfeit of suspects (special section-Type 2 diabetes). Science. 2005;307(5708):3692
  23. Seufert J, Kieffer TJ, Habener JF. Leptin inhibits insulin gene transcription and reverses hyperinsulinemia in leptin-deficient ob/ob mice. Proc. Natl. Acad. Sci. USA. 1999;96(2):674–679
  24. Shao Bao-Mei, Xu Wen, Dai Hui, Tui Pengfei, Li Zhongjun, Gao Xiao-Ming. A study on the immune receptors for polysaccharides from the roots of Astralus membranaceus, a Chinese medicinal herb. Biochem. Biophys. Res. Commun. 2004;320:1103–1111
  25. Surwit RS, Kuhn VM, Cochrane C, et al. Diet-induced type II diabetes in C57BL/6J mice. Diabetes. 1988;37(9):1163–1167
  26. Tomoda M, Shimizu N, Ohara N, Gonda R, Ishii S, Otsuki H. A reticuloendothelial system-activation glycan from the roots of Astragalus membranaceus. Phytochemistry. 1992;31:63–66
  27. Wu F, Chen X. A review of pharmacological study on Astragalus membranaceus (Fisch) Bge. Zhong Yao Cai. 2004;27(3):232–234
  28. Wu Y, Ou Yang JP, Wu K, et al. Hypoglycemic effect of Astragalus polysaccharide and its effect on PTP1B. Acta Pharmacol. Sin. 2005;26(3):345–352
  29. Yang G, Gao X, Yan J, et al. Establishment of GOD-POD assay in a minimal way and application to glucose metabolism of 3T3-L1 adipocyte and HepG2 cell in vitro. Sichuan J. Anat. 2003;11(1):12–15
  30. Ying-Wen Z, Chao-Yan W, Juei-Tang C. Merit of Astragalus polysaccharide in the improvement of early diabetic nephropathy with an effect on mRNA expressions of NF-κB and IκB in renal cortex of streptozotoxin-induced diabetic rats. J. Ethnopharmacol. 2007;114:387–392
  31. Yoshida H, Matsui T, Yamamoto A, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor. Cell. 2001;107(7):881–891

PII: S0944-7113(08)00243-2

doi: 10.1016/j.phymed.2008.12.011

Phytomedicine
Volume 16, Issue 5 , Pages 416-425 , May 2009