ACE inhibition by astilbin isolated from Erythroxylum gonocladum (Mart.) O.E. Schulz

Lucas-Filho, M.D.; Silva, G.C.; Cortes, S.F.; Mares-Guia, T.R.; Perpétua Ferraz, V.; Serra, C.P.; Braga, F.C.

doi:10.1016/j.phymed.2009.09.008

« PreviousPhytomedicine
Volume 17, Issue 5 , Pages 383-387, April 2010

ACE inhibition by astilbin isolated from Erythroxylum gonocladum (Mart.) O.E. Schulz

M.D. Lucas-Filho
- School of Pharmacy, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
G.C. Silva
- Departament of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
S.F. Cortes
- Departament of Pharmacology, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
T.R. Mares-Guia
- Department of Biochemistry and NUCEL – Cell and Molecular Therapy Center, IQ, Universidade de São Paulo, São Paulo, Brazil
V. Perpétua Ferraz
- Department of Chemistry, ICEX, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
C.P. Serra
- School of Pharmacy, Universidade Federal de Ouro Preto, Ouro Preto, Brazil
F.C. Braga
- Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, campus Pampulha, Belo Horizonte, Brazil
- Corresponding author. Tel.: +553134096951; fax: +553134096935.

published online 30 October 2009.

Abstract

Erythroxylum species have several traditional uses in different countries, including the treatment of hypertension. The ethanol extract from E. gonocladum aerial parts, a species endemic to the Brazilian cerrado, elicited a concentration-dependent inhibition of angiotensin converting enzyme (ACE) (pIC₅₀=4.53±0.05). Extract fractionation led to the isolation of two compounds, whose structures were assigned by spectrometric data as astilbin and β-sitosterol, along with a mixture of palmitic, stearic and linolenic acids. This is the first report on the occurrence of these compounds on E. gonocladum. Astilbin promoted significant ACE inhibition in vitro (pIC₅₀=5.86±0.33) and its activity did not differ from captopril, when both compounds were assayed at 10μM concentration.

Keywords: In vitro ACE inhibitory activity, Astilbin, Erythroxylum gonocladum