Topical anti-inflammatory potential of Physalin E from Physalis angulata on experimental dermatitis in mice

Pinto, N.B.; Morais, T.C.; Carvalho, K.M.B.; Silva, C.R.; Andrade, G.M.; Brito, G.A.C.; Veras, M.L.; Pessoa, O.D.L.; Rao, V.S.; Santos, F.A.

doi:10.1016/j.phymed.2010.01.006

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Volume 17, Issue 10 , Pages 740-743, August 2010

Topical anti-inflammatory potential of Physalin E from Physalis angulata on experimental dermatitis in mice

N.B. Pinto
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
T.C. Morais
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
K.M.B. Carvalho
- Departments of Clinical Medicine, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
C.R. Silva
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
G.M. Andrade
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
G.A.C. Brito
- Departments of Morphology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
M.L. Veras
- Department of Organic and Inorganic Chemistry, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
O.D.L. Pessoa
- Department of Organic and Inorganic Chemistry, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
V.S. Rao
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
F.A. Santos
- Departments of Physiology and Pharmacology, Federal University of Ceará, Cel Nunes de Melo 1127, Porangabussu, P.O.B - 3157, 60430-270 Fortaleza, Ceará, Brazil
- Corresponding author. Tel.: +558533668341; fax: +558533668333.

published online 11 February 2010.

Abstract

The anti-inflammatory effect of physalin E, a seco-steroid isolated from Physalis angulata L. was evaluated on acute and chronic models of dermatitis induced by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and oxazolone, respectively, in mouse ear. The changes in ear edema/thickness, production of pro-inflammatory cytokines (TNF-α and IFN-γ), myeloperoxidase (MPO) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. Similar to dexamethasone, topically applied Physalin E (0.125; 0.25 and 0.5mg/ear) potently inhibited the TPA and oxazolone-induced dermatitis, leading to substantial reductions in ear edema/thickness, pro-inflammatory cytokines, and MPO activity. These effects were reversed by mifepristone, a steroid antagonist and confirmed by immunohistochemical and histopathological analysis. The data suggest that physalin E may be a potent and topically effective anti-inflammatory agent useful to treat the acute and chronic skin inflammatory conditions.

Keywords: Physalin E, Physalis angulata, Solanaceae, TPA, Oxazolone, Anti-inflammatory effect