Anti-inflammatory principles from Heritiera littoralis bark

Tewtrakul, S.; Tansakul, P.; Daengrot, C.; Ponglimanont, C.; Karalai, C.

doi:10.1016/j.phymed.2010.02.011

« Previous Next »Phytomedicine
Volume 17, Issue 11 , Pages 851-855, September 2010

Anti-inflammatory principles from Heritiera littoralis bark

S. Tewtrakul
- Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
- Corresponding author. Tel.: +66 74 288888; fax: +66 74 428220.
P. Tansakul
- Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
C. Daengrot
- Department of Chemistry, Faculty of Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
C. Ponglimanont
- Department of Chemistry, Faculty of Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand
C. Karalai
- Department of Chemistry, Faculty of Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand

published online 05 April 2010.

Abstract

Compounds from the hexane, dichloromethane and acetone extracts of Heritiera littoralis bark were investigated for their nitric oxide (NO) inhibitory effects using RAW264.7 macrophage cells. The result indicated that ergosterol peroxide (13) exhibited the highest activity against NO release with an IC₅₀ value of 2.5μM, followed by 6-α-hydroxystigmast-4-en-3-one (11, IC₅₀=9.5μM) and stigmast-4-en-3-one (9, IC₅₀=15.9μM), whereas other compounds showed moderate and mild effects (25.4- > 100μM). Ergosterol peroxide (13) and 6-α-hydroxystigmast-4-en-3-one (11) were also tested against prostaglandin E₂ (PGE₂) and tumor necrosis factor alpha (TNF-α) releases. It was found that ergosterol peroxide (13) possessed marked activity against PGE₂ release with an IC₅₀ value of 28.7μM, while 6-α-hydroxystigmast-4-en-3-one (11) was 86.7μM. However, these two compounds were inactive towards TNF-α release (IC₅₀ > 100μM). The mechanism in transcriptional level of ergosterol peroxide (13) was found to down regulate mRNA expressions of iNOS and COX-2 in dose-dependent manners.