Phytomedicine
Volume 17, Issue 11 , Pages 902-909, September 2010

Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway

  • S. Abbas
    • ,
  • M. Wink

      Affiliations

    • Corresponding Author InformationCorresponding author. Tel.: +49 6221544880; fax: +49 6221544884.

Institute of Pharmacy and Molecular Biotechnology, Department of Biology, Heidelberg University, Im Neuenheimer Feld 364, 69120 Heidelberg, Germany

published online 12 April 2010.

Abstract 

Epidemiological studies have repeatedly demonstrated that green tea protects against oxidative stress involved in many diseases. Health benefits of green tea are attributed to its principal active constituent, epigallocatechin gallate (EGCG). EGCG was shown to increase the stress resistance and lifespan of Caenorhabditis elegans. The mechanism of this action has been investigated in this study. The expression of hsp-16.1 and hsp-16.2 in EGCG-treated worms (N2), as quantified by real-time PCR, was significantly lower under oxidative stress induced by juglone than in controls without EGCG. In the strain TJ356 (DAF-16::GFP) EGCG treatment induced translocation of DAF-16 from the cytoplasm into the nucleus, suggesting that EGCG may affect the daf-2/insulin-like signaling pathway. EGCG decreased the formation of lipofuscin, an aging related pigment. Also, EGCG reduced beta amyloid (Aβ) deposits and inhibited Aβ oligomerization in transgenic C. elegans (CL2006). Thus, the use of green tea and EGCG is apparently rational alternatives for protecting against ROS-mediated and age-related diseases.

Keywords: Caenorhabditis elegans, EGCG, sHSPs, daf-16, Beta amyloid

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PII: S0944-7113(10)00067-X

doi:10.1016/j.phymed.2010.03.008

Phytomedicine
Volume 17, Issue 11 , Pages 902-909, September 2010

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