A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea

Hu, D.D.; Zhang, R.Y.; Zhang, G.Q.; Wang, H.X.; Ng, T.B.

doi:10.1016/j.phymed.2010.07.004

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Volume 18, Issue 5 , Pages 374-379, 15 March 2011

A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea

D.D. Hu
- State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China
R.Y. Zhang
- Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
G.Q. Zhang
- State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China
H.X. Wang
- State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China
- Corresponding author.
T.B. Ng
- School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
- Corresponding author. Tel.: +86 852 2609 8031.

published online 26 August 2010.

Abstract

A laccase, with HIV-1 reverse transcriptase inhibitory activity (IC₅₀=12.7μM) and antiproliferative activity against HepG2 cells (IC₅₀=5.6μM) and MCF7 cells (IC₅₀=6.5μM), was purified from fresh fruiting bodies of the edible white common Agrocybe cylindracea mushroom. The laccase, which had a novel N-terminal sequence, displayed a molecular mass of 58kDa within the range reported for most other mushroom laccases. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, SP-Sepharose, and Q-Sepharose and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but unadsorbed on SP-Sepharose. Its optimum pH was pH 3–4 and its optimum temperature was 50°C. The activity of the isolated laccase differed from one substrate to another. The ranking was ABTS>N,N-dimethyl-1,4-phenylenediamine>hydroquinone>catechol>2-methylcatechol>pyrogallol.