In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.)

Kiyohara, Hiroaki; Ichino, Chikara; Kawamura, Yuka; Nagai, Takayuki; Sato, Noriko; Yamada, Haruki; Salama, Maha M.; Abdel-Sattar, Essam

doi:10.1016/j.phymed.2011.07.004

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Volume 19, Issue 2 , Pages 111-114, 15 January 2012

In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.)

Hiroaki Kiyohara
- Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
- Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan
- Oriental Medicine Research Center, Kitasato University, Tokyo, Japan
Chikara Ichino
- Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
Yuka Kawamura
- Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan
Takayuki Nagai
- Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
- Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan
- Oriental Medicine Research Center, Kitasato University, Tokyo, Japan
Noriko Sato
- School of Pharmacy, Kitasato University, Tokyo, Japan
Haruki Yamada
- Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
- Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan
- Oriental Medicine Research Center, Kitasato University, Tokyo, Japan
Maha M. Salama
- Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
Essam Abdel-Sattar
- Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
- Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
- Corresponding author at: Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt. Tel.: +20 165847211.

published online 07 September 2011.

Abstract

Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-d-glucosyl (1→4)-β-d-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1μg/ml (IC₅₀=0.86μg/ml).